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Home Antibody All anti-SMAD2 antibodies

Anti-SMAD2 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA321257
  • Anti-SMAD2 Rabbit Polyclonal Antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400372) , 20ug Explanation
100ul 325 2-3 Weeks
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WB(1)
IHC(1)
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Also for SMAD2 (NM_001003652)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenPeptide sequence around aa.218~222 (P-E-T-P-P) derived from Human Smad2.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:500-1000
Predicted MW Explanation 52kDa
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens SMAD family member 2 (SMAD2), transcript variant 2
Alternative NamehMAD-2; hSMAD2; JV18; JV18-1; MADH2; MADR2
Database LinkNP_001003652
Entrez Gene 4087 Human
Entrez Gene 17126 Mouse
Entrez Gene 29357 Rat
FunctionTranscriptional modulator activated by TGF-beta and activin type 1 receptor kinase. SMAD2 is a receptor-regulated SMAD (R-SMAD). May act as a tumor suppressor in colorectal carcinoma.
Related PathwayStem cell relevant signaling - JAK/STAT signaling pathwayStem cell relevant signaling - TGFb/BMP signaling pathwayES Cell Differentiation/IPSCancer stem cellsEmbryonic stem cellsTranscription FactorsDruggable Genome Cell cycleWnt signaling pathwayTGF-beta signaling pathwayAdherens junctionPathways in cancerColorectal cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 60 kDa . Positive control: 293 and NIH/3T3 cell lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: 293 cell lysate Lane2: NIH/3T3 cell lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
IHC Image
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded Human breast carcinoma tissue using SMAD2 antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

 

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