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Home Antibody All anti-GAPDH antibodies

Anti-GAPDH Antibody Polyclonal

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319654
  • Rabbit Polyclonal GAPDH Antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419567) , 20ug Explanation
100ug 325 In Stock
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WB(1)
IHC(1)

OriGene Data

ImmunogenGAPDH antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human GAPDH.
Clone NamePolyclonal IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration1mg/ml
Guaranteed Application *WB, IHC Suggested DilutionsELISA, WB: 0.5 - 2 ug/ml, IHC: 10 ug/ml, IF: 20 ug/ml
BufferGAPDH Antibody is supplied in PBS containing 0.02% sodium azide.
Purification GAPDH Antibody is affinity chromatography purified via peptide column.

Reference Data

Target NameHomo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), transcript variant 2
Alternative NameG3PD; GAPD; HEL-S-162eP
Database LinkNP_001243728
Entrez Gene 2597 Human
Entrez Gene 14433 Mouse
Entrez Gene 24383 Rat
FunctionGAPDH Antibody: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. Recent evidence suggests that it also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export. GAPDH has also been implicated in playing a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Related PathwayES Cell Differentiation/IPS Glycolysis / GluconeogenesisMetabolic pathwaysAlzheimer's disease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of GAPDH in multiple cell and tissue lysates with GAPDH antibody at 0.5 ?g/ml. Lanes 1-8, A431, Daudi, HepG2, HL60, Jurkat, human kidney, mouse lung, and chicken liver, respectively.
IHC Image
Immunohistochemical staining of GAPDH in HeLa cells at a dilution of 10 ug/mL.

 

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