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Anti-Esrp2 Antibody 22C5.H7.A6


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  • Mouse monoclonal Esrp-2 antibody
100ug $325 3-7 Days
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OriGene Data

ImmunogenAnti-Esrp-2 is produced by repeated immunizations of full length recombinant mouse Esrp-2 fusion protein.
Clone Name22C5.H7.A6 Isotype
Species ReactivityMouse Concentration1.0 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:100,000, WB: 1:1000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Reference Data

Target NameMus musculus epithelial splicing regulatory protein 2 (Esrp2)
Alternative Name9530027K23Rik; Rbm35b
Database LinkNP_789808
Entrez Gene 77411 Mouse
Related Pathway

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WB Image
Anti-ESRP2 by western blot shows detection of ESRP2 in transfected 293T cell extracts (lane 2). Lanes G and 1 contain 5ug GFP-transfected- and ESRP1-transfected 293T cell lysates, respectively. Briefly, each lane contains approximately 5 µg of lysate. Primary antibody was used at a 1:1000 dilution (PBS-T plus milk) and reacted for O/N at 4C. The membrane was washed and reacted with a 1:10,000 dilution of an anti-mouse ECL antibody for 1hr at room temperature. The bands shown are full length FLAG-ESRP2 (~80kDa) and a slightly lower band that is specific to ESRP2. Molecular weight estimation was made by comparison to prestained MW markers.


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