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Anti-Ido2 Antibody 1HC
Also for Ido2 (NM_145949)
|Anti-IDO2 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with complete mouse IDO2 protein followed by hybridoma development.|
||ELISA: 1:5000-1:50000, WB: 1:500-1:1500, IF: 1:50-1:100
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Indoleamine 2,3-dioxygenase 2 (IDO2) is related to IDO1, and may play a role in T and B Cell Immunity. IDO2’s role in immune regulation will be become clearer following the completion of genetic knock-out studies. IDO2 is expressed in a few select cell types and levels of protein expression appear to be very low, perhaps below the level of detection by IHC. Small-molecule inhibitors of indoleamine 2,3-dioxygenase (IDO) are currently being translated to clinic for evaluation as cancer therapeutics.
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Western Blot of Mouse Anti-IDO2 antibody. Lane 1: HEK293 no transgene cell extracts. Lane 2: mouse IDO-2 transgene expressed in 293HEK. Lane 3: mouse IDO-1 transgene expressed in 293HEK. Primary antibody: IDO2 monoclonal Antibody. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 44.4 kDa, ~60 kDa for IDO 2. Other band(s): IDO2 splice variants and isoforms.