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Anti-EBI3 Antibody V1.4H6.29
Also for EBI3 (NM_015766)
|Anti-EBI-3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.|
|WB, ELISA, IP
||ELISA: 1:10,000, WB: 1:1000, IP: 1ug/mL
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-27 p28 or IL-12 p35 subunit. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R.
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Western blot of Anti-Mouse EBI-3 Antibody. Supernatants from cultures containing indicated cells were immunoprecipitated with an anti-p35 monoclonal plus protein G beads, resolved by SDS page gel and blotted with biotinylated anti mouse-EBI-3 clone V1.4H6.29 at 1 µg/mL. The controls were supernatants from 293T cells transfected with either vector only (negative) or vector containing murine IL-35 (positive).
ELISA assay of EBI-3 Antibody. Supernatant from 293T cells transfected with a vector containing murine IL-35 was added to an ELISA plate coated with V1.4H6.29 (210-301-C55) at 2ug/mL in PBS. Mouse EBI-3 was detected with biotinylated V1.4C4.22 (210-301-B66).
Immunoprecipitation of Mouse Anti-EBI-3 Antibody. Supernatant from 293T cells transfected with a vector containing murine IL-35 was immunoprecipitated with indicated anti-EBI-3 monoclonal clones V1.4H6.29, V1.4C4.22, and V1.4F5.25 plus Protein G beads, resolved by SDS page gel, and blotted with biotinylated anti-mouse EBI-3 clone V1.4H6.29 at 1µg/mL.