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Anti-Actb Antibody 3H2.E4.E12
Also for Actb (NM_007393)
|Anti-beta Actin (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with ß-Actin peptide near the N-Terminus followed by hybridoma development.|
|human, mouse, rat, rabbit, swine
||ELISA: 1:5000-1:50000, WB: 1 ug/mL, IHC: User Optimized
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Antibodies to loading controls are used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.
|Mus musculus actin, beta (Actb)|
|Actx; beta-actin; E430023M04Rik|
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Western Blot of Mouse anti-N-Term Beta Actin Monoclonal Antibody. Lane 1: HeLa Whole Cell Lysate. Lane 2: NIH/3T3 Whole Cell Lysate. Load: 10 µg per lane. Primary antibody: N-Term Beta Actin Monoclonal Antibody at 1.13 ug/mL overnight at 4°C. Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 42 kDa, 42 kDa for N-Term Beta Actin. Other band(s): None.
Immunohistochemistry of Mouse anti-Beta Actin N-Term antibody. Tissue: human spleen. Fixation: formalin fixed paraffin archival tissues. Antigen retrieval: not required. Primary antibody: Beta Actin N-Term antibody at 2.5 µg/mL for 1 h at RT. Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT. Localization: membranous and cytoplasmic. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.