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Home Antibody All anti-BIN1 antibodies

Anti-BIN1 Antibody 99D

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SKU Description Amount Price Availability*  
TA319582 Mouse monoclonal anti-BIN1 antibody 100ug $325 3-7 Days
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WB(1)
FC(1)

OriGene Data

ImmunogenAnti-BIN1 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with a fragment portion of recombinant human BIN1 protein followed by hybridoma development.
Clone Name99D Isotype
Species Reactivitymouse, human Concentration1.0 mg/mL
Guaranteed Application *WB, FC Suggested DilutionsELISA: 1:5000-1:50000, WB: 1:500-1:1500, IHC: 1:100-1:500, IP: 10-100 uL, FC: 0.5-1x10^6 cells
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Bin1 is a conserved member of the BAR family of genes that have been implicated in diverse cellular processes including endocytosis, actin organization, programmed cell death, stress responses, and transcriptional control. The first mammalian BAR protein to be discovered, Amphiphysin I (AmphI), was identified in an immunoscreen for proteins associated with the plasma membranes of synaptic neurons, functions in the control of clathrin-dependent synaptic vesicle endocytosis. The mammalian Bin1 gene was first identified in a two hybrid screen for polypeptides that bind to the N-terminal Myc box 1 (MB1) portion of the c-Myc oncoprotein. Bin1 is similar to AmphI in overall structure, with an N-terminal BAR domain and a C-terminal SH3 domain. However, the Bin1 gene is more complex than the AmphI gene, encoding at least seven different splice variants that differ widely in subcellular localization, tissue distribution, and ascribed functions. Alternate splicing of the Bin1 gene results in ten transcript variants encoding different isoform. Bin1 is expressed ubiquitously in mammalian cells. Certain splice variants of Bin1 are expressed in the neurons, muscle cells or tumor cells. Bin1 may act as a cancer suppressor and inhibits malignant cell transformation. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Bin1 has also been implicated in Alzheimer disease and cardiac disease. Defects in Bin1 are the cause of centronuclear myopathy autosomal recessive; also known as autosomal recessive myotubular myopathy.

Reference Data

Target NameHomo sapiens bridging integrator 1 (BIN1), transcript variant 8
Alternative NameAMPH2; AMPHL; SH3P9
Database LinkNP_004296
Entrez Gene 274 Human
Entrez Gene 30948 Mouse
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western Blot of Anti-BIN1 Antibody. Lane 1: Keratinocyte derived from Bin1 wild type mice. Lane 2: Keratinocyte derived from Bin1 null mice. Load: 35 µg per lane. Primary antibody: BIN1 monoclonal Antibody. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 1xPBS, 0.4% Tween-20. Other band(s): non-specific.
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Flow Cytometry of Mouse Anti-BIN1 Antibody. Cells: C2C12 cells. Stimulation: none. Primary antibody: Anti-IgD (control), Anti-BIN-1 Antibody (99D clone). Secondary antibody: Biotin mouse secondary antibody at 1:10,000 for 45 min at RT and streptavidin PE at 1:5,000 for 30 min at RT.
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