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Anti-STAT5A PHOSPHO Antibody 5F6.F1


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SKU Description Amount Price Availability*  
TA319572 Mouse monoclonal STAT5 phospho Y694 antibody (Phospho-specific) 100ug $356 3-7 Days
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OriGene Data

ImmunogenAnti-STAT5 phospho Y694 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.
Clone Name5F6.F1 Isotype
Species Reactivityhuman, mouse, rat Concentration1.0 mg/mL
Guaranteed Application *WB, IHC, IF Suggested DilutionsELISA: 1:20,000, WB: 1:500 - 1:2,000, IHC: 20-40 ug/ml, IF: 1:50-1:1000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Signal transducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic transcription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5a ?and Stat5ß. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus. Ideal for Cancer, Chromatin & Nuclear Signaling and Signal Transduction research.

Reference Data

Target NameMus musculus signal transducer and activator of transcription 5A (Stat5a), transcript variant 1
Alternative NameAA959963; STAT5
Database LinkNP_035618
Entrez Gene 6776 Human
Entrez Gene 20850 Mouse
Entrez Gene 24918 Rat
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
WB using anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4?. For detection DyLight™800 conjugated Gt-a-Mouse IgG was used at a 1:20,000 dilution for 30 min at RT.

IHC Image
IHC using Anti-Stat5 pY694 monoclonal antibody shows detection of phosphorylated Stat5pY694 in human breast tissue (40X). The antibody was used a dilution to 20 µg/mL. The image shows breast epithelium with moderate nuclear staining. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.

IF Image
Anti-Stat5 pY694 (MOUSE) Monoclonal Antibody (1:500) detecting Stat5 in 3T3 cells (immunofluorescent STED microscopy). Red represents Stat5 pY694 protein. Green represents tubulin.


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