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Home Antibody All anti-PMS2 antibodies

Anti-PMS2 Antibody 349.29.5.2

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SKU Description Amount Price Availability*  
TA319565
  • Mouse monoclonal anti-PMS2 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC424658) , 20ug
100ug 325 3-7 Days
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WB(1)
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Also for PMS2 (NM_000535)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human PMS2 corresponding to the first 133 amino acid residues of the protein. The clone was produced using conventional hybridoma technology.
Clone Name349.29.5.2 Isotype
Species Reactivityhuman, mouse, rat, hamster, chimpanzee Concentration1.38 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note PMS2 is a highly conserved nuclear protein involved in mismatch repair during DNA replication and has been identified to be composed as a heterodimer of PMS2 and MLH1.  PMS is part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4), Turcot syndrome (an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas) and supratentorial primitive neuroectodermal tumors with cafe-au-lait spots (SNTCL). The human PMS2 gene encodes an 862 aa, 96 kDa polypeptide.

Reference Data

Target NameHomo sapiens PMS2 postmeiotic segregation increased 2 (S. cerevisiae) (PMS2), transcript variant 1
Alternative NameHNPCC4; PMS2CL; PMSL2
Database LinkNP_000526
Function
Related Pathway

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WB Image
Western blot analysis is shown using Protein A Purified Mouse Monoclonal Anti-PMS2 antibody to detect human PMS2 protein present in H157 cell lysates. Approximately 5,10 and 30 ug of cell lysate was loaded on a 4-12% NuPage SDS-PAGE gel using MES buffer. The blot was incubated with a 1:1,000 dilution of the antibody at room temperature followed by washing. A 1:20,000 dilution of HRP conjugated Gt-anti-Mouse IgG preceded color development using Pierce Chemical's SuperSignal™ substrate. Comparison to a molecular weight marker (not shown) indicates a single band of ~96.0 kDa corresponding to the expected molecular weight for human PMS2 protein. Other detection systems will yield similar results. Personal communication Morphotek Inc.

 

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