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Home Antibody All anti-AKT1 antibodies

Anti-AKT1 Antibody 17F6.B11

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SKU Description Amount Price Availability*  
TA319563
  • Mouse monoclonal Akt phospho S473 antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC423056) , 20ug
50ug $325 3-7 Days
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WB(1)
IHC(2)
ELISA(1)
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Also for AKT1 (NM_001014431)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenAnti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
Clone Name17F6.B11 Isotype
Species Reactivityhuman, mouse, rat, monkey Concentration1.0 mg/mL
Guaranteed Application *WB, ELISA, IHC Suggested DilutionsELISA: 1:20,000, WB: 1:500-1:3,000, IHC: 20 ug/mL, IF: 1:500-1:3,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note AKT phospho 473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 3
Alternative NameAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Database LinkNP_001014431
Function
Related Pathway
Apoptosis
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
Hemostasis
Jak-STAT signaling pathway
MAPK signaling pathway
Signaling by GPCR
Toll-like receptor signaling pathway
Wnt Signaling Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western Blot of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: none. Load: 25 ng per lane. Primary antibody: Akt phospho S473 Biotin Conjugated antibody at 1:1,000 for overnight at 4°C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 79 kDa, 79 kDa for Akt phospho S473. Other band(s): none
ELISA Image
ELISA of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Antigen: BSA conjugates of Akt phospho S473 and AKT non-phospho S473. Coating amount: 0.1 µg per well. Primary antibody: Akt phospho S473 Biotin Conjugated antibody at 5 µg/mL. Dilution series: 3-fold. Mid-point concentration: 5 ng/mL Akt phospho S473 Biotin Conjugated antibody. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:10,000. Substrate: TMB (p/n TMBE-0100)
IHC Image
Immunohistochemistry of mouse anti AKT phospho S473 biotin conjugated at 40X (left) with negative control (right) Tissue: prostate Fixation: FFPE buffered formalin 10% conc Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker Primary antibody: 20 µg/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.
IHC Image
Immunohistochemistry of mouse Anti-AKT pS473 (MOUSE) Biotin Conjugated  at 40X Tissue: prostate Fixation: FFPE buffered formalin 10% conc Antigen retrieval: Heat, Citrate pH 6.2. Pressure Cooker (pH 9 shown on right as negative control) Primary antibody: 20 µg/mL for 1 h at RT Secondary antibody: Streptavidin Conj. HRP 10 ug/ml Localization: nuclear and occasionally cytoplasmic Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

 

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