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Home Antibody All anti-AKT1 antibodies

Anti-AKT1 Antibody 18F10.E5


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA319560 Mouse monoclonal anti-AKT antibody 100ug $325 In Stock
LC423056 AKT1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for AKT1 (NM_001014431)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenAnti-AKT Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues near the C terminal end of of human AKT1 protein.
Clone Name18F10.E5 Isotype
Species Reactivityhuman, mouse, rat, chimpanzee Concentration1.0mg/ml
Guaranteed Application *WB Suggested DilutionsELISA: 1:20,000, WB: 1:500 - 1:3,000, IHC: 20 ug/mL
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphory-lates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 3
Database LinkNP_001014431
Entrez Gene 207 Human
Entrez Gene 11651 Mouse
Entrez Gene 24185 Rat
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >>

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* OriGene provides validated application data and protocol, with money back guarantee.

WB of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. Lane 2: PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG (p/n 610-103-121) was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax™ enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Block: 5% BLOTTO (p/n B501-0500 in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.


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