OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-AKT1 antibodies

Anti-AKT1 Antibody 18F3.H11

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319559
  • Mouse monoclonal Akt phospho pT308 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC423056) , 20ug Explanation
100ug 325 3-7 Days
Add to Shopping Cart
spacer
WB(1)
IHC(1)
spacer
Also for AKT1 (NM_001014431)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenAnti-AKT pT308 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein.
Clone Name18F3.H11 Isotype
Species Reactivityhuman, mouse, rat, monkey Concentration1.0 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:20,000, WB: 1:500 - 1:3,000, IHC: 20 ug/ml
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Akt1 pT308 antibody detects phosphorylated Akt. AKT is involved in cellular survival pathways, by inhibiting apoptotic processes. Akt is also able to induce protein synthesis pathways, and is therefore a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein with 3 isoforms known as AKT1, AKT2, AKT3. AKT is also known as protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phos-phorylated, AKT dissociates from the membrane and phos-phorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT pT308 monoclonal antibody is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, Signal Transduction research.

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 3
Alternative NameAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Database LinkNP_001014431
Entrez Gene 207 Human
Entrez Gene 11651 Mouse
Entrez Gene 24185 Rat
Entrez Gene 697747 Monkey
Function
Related PathwayProtein KinaseES Cell Differentiation/IPSDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwaymTOR signaling pathwayApoptosisVEGF signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western Blot of Mouse anti-AKT pT308 antibody. Lane 1: non-phosphorylated AKT in untreated cells . Lane 2: phosphorylated AKT on PDGF stimulated NIH/3T3 cell lysates . Load: 15 µg per lane. Primary antibody: AKT pT308 antibody at a 1:4,000 dilution in TBS with 3% BSA, for 3 h at 4° C. Secondary antibody: peroxidase conjugated Gt-a-Mouse IgG (Fc) (p/n 610-1303) was used at a 1:40,000 dilution for 1 h at 4° C. Block: 3% BSA (p/n BSA-30) in TBS for 30 min at RT. Predicted/Observed size: (indicated by arrowhead at ~56 kDa). Other band(s): unspecific.
IHC Image
Immunohistochemistry of Mouse anti-AKT pT308 antibody. Tissue: human brain cerebellum tissue (40X). Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT pT308antibody at 20 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: staining of Purkinje neurons and cell processes in the cerebellum, cytosolic as well as occasionally nuclear. Staining: AKT pT308 as precipitated red signal with hematoxylin purple nuclear counterstain.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title