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Home Antibody All anti-AKT1 antibodies

Anti-AKT1 Antibody 17F6.B11

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SKU Description Amount Price Availability*  
TA319558
  • Mouse Balb/c monoclonal Akt phospho S473 ATTO594 Conjugated antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC423056) , 20ug
100ug $325 In Stock
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WB(2)
IF(1)
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Also for AKT1 (NM_001014431)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenAkt phospho S473 ATTO594 Conjugated Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
Clone Name17F6.B11 Isotype
Species Reactivityhuman, mouse, rat, monkey Concentration0.82mg/ml
Guaranteed Application *WB, IF Suggested DilutionsELISA: 1:20,000, WB: >1:10,000, IF: >1:5,000, ELISA: >1:20,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Anti-AKT antibody detects AKT which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Phospho AKT antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 3
Alternative NameAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Database LinkNP_001014431
Function
Related Pathway
Apoptosis
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
Hemostasis
Jak-STAT signaling pathway
MAPK signaling pathway
Signaling by GPCR
Toll-like receptor signaling pathway
Wnt Signaling Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western Blot of Mouse Anti-Akt pS473 antibody. Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated Akt1, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated Akt1 (red band). Load: 10 µg per lane. Primary antibody: rabbit anti-Akt (pan) and mouse anti-Akt pS473 specific antibodies at 1:400 for overnight at 4°C. Secondary antibody: DyLight™ 549 conjugated anti-rabbit IgG (green) and DyLight™ 649 conjugated anti-mouse IgG (red) secondary antibodies at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
WB Image
Western Blot of Mouse anti-AKT pS473 antibody Atto 594 Conjugated. Lane 1: GST Tagged AKT 1 Active Recombinant Protein. Lane 2: None. Load: 25 ng per lane. Primary antibody: None. Secondary antibody: Atto 594 mouse secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: ~100 kDa, ~100 kDa for AKT pS473. Other band(s): None.
IF Image
ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.

 

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