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Anti-BIN3 Antibody 3A4
Also for BIN3 (NM_018688)
|Anti-BIN3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with a full legnth human BIN3 recombinant protein followed by hybridoma development.|
||ELISA: 1:5000-1:50000, WB: 1:500-1:1500, IP: 10-100 uL
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|BIN-3 encodes an evolutionarily conserved and ubiquitously expressed member of the BAR superfamily of curved membrane and GTPase-binding proteins implicated in signal transduction and vesicular trafficking. In humans, Bin3 maps to chromosome 8p21.3, a region widely implicated in cancer suppression that is often deleted in non–Hodgkin’s lymphomas and various epithelial tumors. Study in mouse suggests that homozygous inactivation of Bin3 causes cataracts and an increased susceptibility to lymphomas during aging.
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Western Blot of Mouse Anti-BIN3 antibody. Lane 1: MEF cells wild type. Lane 2: MEF cells null mice. Load: 35 µg per lane. Primary antibody: BIN3 antibody overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 29.7 kDa, 30 kDa for BIN-3. Other band(s): non-specific.
Western Blot of Mouse anti-BIN3 antibody. Lane 1: MEF lysate BIN3 +/-. Lane 2: MEF lysate BIN3 -/-. Load: 35 µg per lane. Primary antibody: BIN-3 antibody at 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ mouse secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 29.7 kDa, 31 kDa for BIN 3. Other band(s): * non-specifics.