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Home Antibody All anti-GDF15 antibodies

Anti-GDF15 Antibody 23B3D2.H5

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA319547 Mouse monoclonal anti-NAG-1 antibody 100ug $325 3 Weeks
LC401529 GDF15 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
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Also for GDF15 (NM_004864)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenThis Protein A purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminal end of human NAG-1 protein.  A residue of cysteine was added to facilitate coupling to KLH.
Clone Name23B3D2.H5 Isotype
Species ReactivityHuman Concentration1.13 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:2,000, WB: 1:1,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Non-steroidal anti-inflammatory drug (NSAID) activated gene (NAG-1) is a member of the transforming growth factor-beta (TGF-beta) superfamily. NAG-1 is also known as Macrophage Inhibitory Cytokine-1 (MIC-1), Growth Differentiation Factor 15 (GDF15), Placental Bone Morphogenetic Protein (PLAB), or Prostate Derived Factor (PDF). NAG-1 is expressed in human placenta, prostate and colon. It possesses antitumorigenic and proapoptotic activities. NAG-1 expression is dramatically increased in inflammation, injury and malignancy. Increase of NAG-1 expression is a feature of many cancers including breast, colon, pancreas and prostate. In a number of studies, NAG-1 expression was increased by a number of NSAIDs. This increase in expression may correlate with the chemopreventive effect NSAIDs seem to have with certain cancers. NAG-1 expression is also induced by PPAR gamma ligands and by several dietary compounds such as conjugated linoleic acids (CLAs), naturally occurring fatty acids in ruminant food products, indoles, epicatechin gallate, and genistein. Induced expression of NAG-1 results in stimulation of apoptosis and inhibition of cell growth. Inhibition of NAG-1 induced expression by small interference RNA (siRNA) results in repression of induced apoptosis. NAG-1 expression is regulated by a numbers of transcription factors such as ERG-1 and Sp1. EGR-1 may be necessary for NSAID-induced NAG-1 expression. The study of expression of NAG-1 proteins, including variants, is important to define their potential role as serum biomarkers for cancer diagnosis, treatment monitoring, epidemiology study, and nutrition surveys.

Reference Data

Target NameHomo sapiens growth differentiation factor 15 (GDF15)
Alternative NameGDF-15; MIC-1; MIC1; NAG-1; PDF; PLAB; PTGFB
Database LinkNP_004855
Entrez Gene 9518 Human
Function
Related PathwaySecreted ProteinDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB shows detection of recombinant NAG-1 protein present in Pichia pastoris whole cell lysates: lane 1 - yeast cell lysate expressing NAG-1 H variant with SUMO expression tag at 36 kDa; lane 2 - yeast cell lysate expressing NAG-1 D variant with SUMO expression tag at 36 kDa; lane 3 - yeast cell lysate expressing NAG-1 H variant; and lane 4 - yeast cell lysate expressing NAG-1 D variant. Primary antibody was used at 1:1,000. HRP conjugated Gt-a-Mouse IgG secondary antibody was used AT 1:40000.
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