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Anti-INS Antibody 2D11.H5
|This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA).|
||ELISA: 1:5,000 - 1:25,000, WB: 1:50 - 1:200, IHC: 1:50 - 1:200
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Recognizes the 51 amino acid (6 kDa) insulin polypeptide composed of A and B chains. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-peptide basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain acids. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids. The main storage site for insulin is the pancreatic islets. Antibodies to insulin are important as b-cell and tumor (insulinoma) markers).
|Homo sapiens insulin (INS), transcript variant 1|
|IDDM2; ILPR; IRDN; MODY10|
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ELISA Results of Mab anti-Insulin antibody tested against human insulin by ELISA. Each well was coated with 0.1?g of conjugate. The starting concentration of antibody in the dilution series was 10 ?g/ml. Each point on the Y-axis represents a 3-fold dilution. The midpoint of the titration curve represents approx-imately 5ng/ml antibody or a 1:200,000 dilution from the stock concentration. HRP conjugated Gt-a-Mouse IgG H&L(p/n 610-103-121) and TMB substrate were used for detection.
Dot blotting. Mab anti-Insulin antibody (clone 2D11.H5) is shown to detect human insulin by dot blot. Each dot blot represents 1 µl of non-denatured human insulin at various dilutions starting at 1.0 µg/ml spotted on to nitrocellulose. A 1:400 dilution of Mab anti-Insulin is used for 2 hour followed by detection using a 1:5,000 dilution of IRDyeTM800 conjugated Goat-a-Mouse IgG [H&L] (610-132-121) reacted for 45 min at room temperature and visualization using the Odyssey® Infrared Imaging System developed by LI-COR. Other detection systems will yield similar results. IRDye is a trademark of LI-COR, Inc.