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Home Antibody All anti-FBXO4 antibodies

Anti-FBXO4 Antibody

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SKU Description Amount Price Availability*  
TA319513 Rabbit polyclonal anti-FBXO4 antibody 100ul $325 3-7 Days
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WB(1)
IF(1)

OriGene Data

ImmunogenThis whole rabbit serum was prepared by repeated immunizations with a synthetic peptide derived from sequences unique to the N-terminus of FBX4 and conserved between the human and murine proteins (see link below for the full-length sequence of the mouse gene product).
Clone Name IsotypeIgG
Species Reactivityhuman, mouse Concentration85 mg/mL
Guaranteed Application *WB, IF Suggested DilutionsELISA: 1:5,000 - 1:25,000, WB: 1:500 – 1:3000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Skp/Cullin/F-box (SCF) complexes are part of a large family of E3 ubiquitin ligases in which F-box proteins determine substrate specificity.  Substrate selection by F-box proteins may also be aided by accessory cofactors.  The F-box only protein 4, FBX4, interacts via its F-box with SKP1 and CUL1 to form SCFFBX4 ubiquitin ligase.  The activity of the SCFFBX4 complex can be regulated by aB crystallin, a small heat shock protein which serves as a specificity cofactor for FBX4 dependent ubiquitination.  FBX4 together with aB crystallin (SCFFBX4-aB crystallin) promotes the ubiquitination of cyclin D1 phosphorylated at Thr-286.  In addition, the    SCFFBX4-aB crystallin ubiquitin ligase has been implicated in the ubiquitination of unknown substrates in desmin-related myopathy.  SCFFBX4, in the absence of aB crystallin, also ubiquitinates the telomeric protein PIN2/TRF1.

Reference Data

Target NameMus musculus F-box protein 4 (Fbxo4)
Alternative Name1700096C12Rik; AI851261; AW494535; Fbx4
Database LinkNP_598860
Entrez Gene 26272 Human
Entrez Gene 106052 Mouse
Function
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

Whole cell extracts prepared from U2OS cells, or insect Sf9 cells expressing Flag-FBX4, were resolved by SDS-PAGE and transferred to nitrocellulose. FBX4 was detected using Rockland’s anti-FBX4 at a 1:500 dilution, in TBS buffer containing 0.1% Tween-20, followed by peroxidase conjugated anti-rabbit IgG. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA.
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Immunocytochemistry staining of NIH-3T3 cells with Rockland’s anti-FBX4. Cells were fixed in methanol/acetone followed by incubation with anti-FBX4 at a 1:300 dilution. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia,
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