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Home Antibody All anti-RNF25 antibodies

Anti-RNF25 Antibody

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SKU Description Amount Price Availability*  
TA319495 Rabbit polyclonal anti-RNF25 antibody 100ug $325 3-7 Days
LC411668 RNF25 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IP(1)
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Also for RNF25 (NM_022453)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse Concentration1.0 mg/mL
Guaranteed Application *WB, IP Suggested DilutionsELISA: 1:10,000 - 1:40,000, WB: 1:200 - 1:2,000, IP: 1 mg
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues.  RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait.  RNF25 has also been shown to act as a putative E3 ligase, at least in vitro.  RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kB by interacting with p65 in vivo upon stimulation with TNF.  Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates.  Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD).

Reference Data

Target NameHomo sapiens ring finger protein 25 (RNF25)
Alternative NameAO7
Database LinkNP_071898
Entrez Gene 64320 Human
Entrez Gene 57751 Mouse
Function
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot using affinity purified anti-RNF25 antibody shows detection of RNF25 (arrow head) in HEK293 cells over-expressing human RNF25 (lane 1) or mouse RNF25 (lane 2). Lane 1 is a vector only control. The extracts were loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with the primary antibody diluted to 1:1,000. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.
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1 µg of Rockland’s affinity purified anti-RNF25 was used in immunoprecipitation of 20 µg HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti-HA tag antibody. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD.
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