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Home Antibody All anti-WRNIP1 antibodies

Anti-WRNIP1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319493
  • Rabbit polyclonal anti-WHIP antibody
100ug 325 3-7 Days
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WB(2)
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Also for WRNIP1 (NM_020135)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of the WHIP1 protein.  The immunogen sequence shows 100% homology to human WHIP1 (isoform 1) and WHIP2 (isoform 2) with predicted molecular weights of 72.2 kDa and 69.5 kDa, respectively.  The immunogen sequence also shows 100% homology to WHIP1 from mouse, rat and monkey sequences.  Reactivity with WHIP proteins from other sources is not known, but is likely due to reported homologies.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat, monkey Concentration1.10 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:10,000 - 1:40,000, WB: 1:500 - 1:2,000, IHC: User Optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Werner's syndrome is a rare autosomal recessive disorder characterized by premature aging.  Werner helicase interacting protein 1 (WHIP) interacts with the N-terminal portion of Werner protein, which contains an exonuclease domain. This protein shows homology to replication factor C family proteins, and is conserved from E. coli to human. Studies in yeast suggest that this gene product may influence the aging process.  A second isoform exists (WHIP2).

Reference Data

Target NameHomo sapiens Werner helicase interacting protein 1 (WRNIP1), transcript variant 1
Alternative NamebA420G6.2; WHIP
Database LinkNP_064520
Function
Related Pathway

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WB Image
Western blot analysis is shown using Affinity Purified anti-Human WHIP antibody to detect Human WHIP present in a HEK293 whole cell lysate.  ~30µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected.  The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein.  See Figure 2 for the results of peptide competition experiments. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 (611-132-122) diluted 1:5,000 for 45 min.  IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.
WB Image
Western blot analysis is shown using anti-Human WHIP antibody with and without pre-incubation with blocking peptide.  Testing was performed on antiserum prior to affinity purification.  Peptide competition (left) blocks the specific staining, whereas the control (right) shows staining of a strong dominant band corresponding to human WHIP1.  ~30µg of HEK293 lysate was loaded per lane for 4-20% gradient SDS-PAGE.  Comparison to a molecular weight marker (not shown) indicates a band of ~96.0 kDa is detected. The blot was incubated with a 1:1000 dilution of the antibody at room temperature for 2 h followed by detection using IRDye® 800 labeled Goat-a-Rabbit IgG [H&L] MX10 (611-132-122) diluted 1:5,000 for 45 min.  IRDye® 800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other systems will yield similar results.

 

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