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Anti-DLL4 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319489
  • Rabbit polyclonal anti-DELTA-4 antibody
100ug $325 In Stock
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WB(1)
IHC(1)
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Also for DLL4 (NM_019074)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenAnti-DELTA-4 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human DELTA-4.
Clone Name IsotypeIgG
Species Reactivityhuman, partial reactivity to mouse and rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:2,500, IHC: 10 ug/mL to 20 ug/mL
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note DELTA-4 (also called delta 4 precursor, delta ligand 4 precursor, Delta-like protein 4, DLL4, Drosophila Delta homolog 4 and notch ligand DLL4 precursor) is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.  DELTA-4  plays a role in the Notch signaling pathway and activates Notch-1 and Notch-4. DELTA-4 is a membrane protein that is expressed in the vascular endothelium.

Reference Data

Target NameHomo sapiens delta-like 4 (Drosophila) (DLL4)
Alternative Namehdelta2
Database LinkNP_061947
Function
Related Pathway
Delta-Notch Signaling Pathway

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WB Image
Western Blot of Rabbit anti-Delta-4 antibody. Lane 1: mouse pancreatic tissue. Load: 20 µg per lane. Primary antibody: Delta-4 antibody at 1:500 for overnight at 4°C. Secondary antibody: HRP-labeled Goat anti-Rabbit IgG secondary antibody at 1:5,000 for 1 hour at RT. A chemiluminescence system was used for signal detection using a 3 min exposure time. Block: 5% Goat Serum overnight at 4°C. Predicted/Observed size: 74 kDa, 70 kDa for Delta 4. Other band(s): none.
IHC Image
Affinity Purified anti-Delta-4 antibody was used at 20 µg/ml to detect Delta-4 in a variety of tissues including colon, liver, skeletal muscle, ovary, pancreas, prostate, testes, thymus, tonsil and uterus. In contrast to reported findings, no staining was observed in vascular tissue. This image shows Delta-4 staining of human ovary. Tissue was formalin-fixed and paraffin embedded. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.

 

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