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Home Antibody All anti-POLK antibodies

Anti-POLK Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319484
  • Goat polyclonal anti-POLK antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC414115) , 20ug Explanation
100ug 325 3-7 Days
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WB(1)
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Also for POLK (NM_016218)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis affinity-purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).
Clone Name Isotype
Species Reactivityhuman Concentration1.4 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:3,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note POLK (also known as DNA polymerase kappa, DINB protein and DINP) is involved in DNA repair.  POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK lacks 3'-5' proofreading exonuclease activity.  POLK is a nuclear protein that binds to REV1L and PCNA. POLK is detected throughout the nucleus and at replication foci. Multiple alternative splice variants have been detected for this protein. POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.

Reference Data

Target NameHomo sapiens polymerase (DNA directed) kappa (POLK)
Alternative NameDINB1; DINP; POLQ
Database LinkNP_057302
Entrez Gene 51426 Human
Function
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using Affinity Purified anti-POLK antibody shows detection of a band ~98 kDa corresponding to human POLK (arrowhead).  Approximately 35 µg of a HeLa whole cell lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose.   After blocking the membrane was probed with the primary antibody diluted to 1:1,000.  Reaction occurred overnight at 4° followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu (605-432-013) for 45 min at room temperature. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.

 

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