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Home Antibody All anti-EIF3E antibodies

Anti-EIF3E Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319457
  • Rabbit polyclonal Eif3S6 Int6 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400602) , 20ug Explanation
100ug 325 3-7 Days
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WB(2)

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminus of mouse EIF3S6/Int6.
Clone Name IsotypeIgG
Species Reactivitymouse, human, rat, dog, bovine, monkey Concentration0.8 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:90,000, WB: 1:1,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers.  The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system.  Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an amino-terminal truncated gene product.  Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems.  This gene product plays a role in regulating translation initiation and is a component of the eIF3 translation initiation complex.  There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stress-responsive genes.  Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth.

Reference Data

Target NameMus musculus eukaryotic translation initiation factor 3, subunit E (Eif3e)
Alternative Name48kDa; eIF3-p46; eIF3-p48; Eif3s6; Int6
Database LinkNP_032414
Entrez Gene 3646 Human
Entrez Gene 16341 Mouse
Entrez Gene 299872 Rat
Entrez Gene 697622 Monkey
Entrez Gene 475070 Dog
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6).  The identity of the higher and lower molecular weight bands is unknown.  The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6.  Primary antibody was used at 1:1000. Personal communication, J.Lee, NCI, Bethesda, MD.
WB Image
Western blot using affinity purified anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2. Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used. Personal communication, J.Lee, NCI, Bethesda, MD.

 

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