OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-Il1b antibodies

Anti-Il1b Antibody

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319453
  • Rabbit polyclonal anti-IL1 beta antibody
100ug 325 In Stock
Add to Shopping Cart
spacer
WB(1)
IF(1)
spacer
Also for Il1b (NM_008361)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenThis antibody was prepared by repeated immunizations with recombinant mouse IL-1ß produced in E.coli.  The MW of recombinant mouse IL-1ß was 17 kDa.
Clone Name IsotypeIgG
Species Reactivitymouse and rat Concentration1mg/ml
Guaranteed Application *WB, IF Suggested DilutionsELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000, IHC: 1:50-1:250, IF: 1:50-1:250, IP: 1:200-1:800, FC: User Optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note IL-1 beta (also known as Interleukin-1 beta, IL-1ß and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1ß is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. Anti-IL-1 beta antibody is ideal for investigators involved in Cardiovascular and Immunology research.

Reference Data

Target NameMus musculus interleukin 1 beta (Il1b)
Alternative NameIl-1b; IL-1beta
Database LinkNP_032387
Entrez Gene 16176 Mouse
Entrez Gene 24494 Rat
Function
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1ß containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1ß precursor molecule. Denatured IL-1ß will have a 18 kDa band.
IF Image
Immunofluorescence microscopy after staining of mouse carotid artery tissue with anti-Mouse IL-1ß antiserum (less purified form of TA319453) diluted 1:50. Tissue sections were prepared after cyrofixation. Reaction occurred at room temperature for 60' followed by washes and reaction with Rhodamine conjugated Gt-a-Rabbit IgG (code 611-100-122). Tissue was counterstained with bis-benzimide solution at 0.5 mg/ml in PBS for 15 min at room temperature. Panel A) shows no antibody staining of WT uninjured mouse carotid tissue. Panel B) shows anti-IL-1ß staining of cells after surgical injury of tissue. Panel C) shows no antibody staining of injured carotid tissue from an IL-1ß KO mouse.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title