Home Antibody All anti-Il1b antibodies
Also for Il1b (NM_008361)
|This antibody was prepared by repeated immunizations with recombinant mouse IL-1ß produced in E.coli. The MW of recombinant mouse IL-1ß was 17 kDa.|
|mouse and rat
||ELISA: 1:1,000 - 1:5,000, WB: 1:500 - 1:2,000, IHC: 1:50-1:250, IF: 1:50-1:250, IP: 1:200-1:800, FC: User Optimized
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|IL-1 beta (also known as Interleukin-1 beta, IL-1ß and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1ß is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins. Anti-IL-1 beta antibody is ideal for investigators involved in Cardiovascular and Immunology research.
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This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1ß containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in western blot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1ß precursor molecule. Denatured IL-1ß will have a 18 kDa band.
Immunofluorescence microscopy after staining of mouse carotid artery tissue with anti-Mouse IL-1ÃŸ antiserum (less purified form of TA319453) diluted 1:50. Tissue sections were prepared after cyrofixation. Reaction occurred at room temperature for 60' followed by washes and reaction with Rhodamine conjugated Gt-a-Rabbit IgG (code 611-100-122). Tissue was counterstained with bis-benzimide solution at 0.5 mg/ml in PBS for 15 min at room temperature. Panel A) shows no antibody staining of WT uninjured mouse carotid tissue. Panel B) shows anti-IL-1ÃŸ staining of cells after surgical injury of tissue. Panel C) shows no antibody staining of injured carotid tissue from an IL-1ÃŸ KO mouse.