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Home Antibody All anti-GLI2 antibodies

Anti-GLI2 Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA319437 Rabbit polyclonal anti-Gli-2 antibody 100ug $325 3-7 Days
LC417413 GLI2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 46-60 of human Gli-2 (isoform a).
Clone Name IsotypeIgG
Species Reactivityhuman, chimpanzee Concentration1.1 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:2,000 - 1:12,000, WB: 1:500 - 1:2,000, IHC: 1:500 - 1:2,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Gli-2 (also known as Zinc Finger Protein Gli-2, GLI-Kruppel family member GLI-2 or Tax helper protein) belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila

Reference Data

Target NameHomo sapiens GLI family zinc finger 2 (GLI2)
Alternative NameCJS; HPE9; PHS2; THP1; THP2
Database LinkNP_005261
Entrez Gene 2736 Human
Function
Related PathwayES Cell Differentiation/IPSAdult stem cellsCancer stem cellsEmbryonic stem cellsDruggable Genome Hedgehog signaling pathwayPathways in cancerBasal cell carcinoma

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB using Anti-Gli-2 antibody shows detection of Gli-2 protein in rat testes (lane 1) and human HEK293 (lane 2) whole cell lysates (arrowhead). Each lane contains approximately 35 µg of lysate.  Primary antibody was used at a 1:400 dilution.  The membrane was washed and reacted with a 1:10,000 dilution of IRDye® 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 for 45 min at RT.   Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).
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