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Home Antibody All anti-EIF3F antibodies

Anti-EIF3F Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA319432 Rabbit polyclonal anti-EIF3F antibody 100ug $356 3-7 Days
LC418450 EIF3F HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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Also for EIF3F (NM_003754)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 114-125 of human eIF3f protein.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat, chimpanzee, chicken, bovine Concentration1.0 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:10,000 - 1:40,000, WB: 1:200 - 1:2,000, IHC: User Optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note eIF3f, also known as eukaryotic translation initiation factor 3 subunit 5, eIF-3 epsilon, and eIF3 p47 subunit, binds to the 40S ribosome and promotes the binding of methionyl-tRNA and mRNA. EIF3f also associates with the complex p170-eIF3. eIF-3 is composed of at least 12 different subunits, eIF3f is one of these subunits.

Reference Data

Target Nameeukaryotic translation initiation factor 3 subunit F
Alternative NameEIF3S5|eIF3-p47
Database LinkNP_003745
Entrez Gene 8665 Human
Entrez Gene 66085 Mouse
Entrez Gene 293427 Rat
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using affinity purified anti-eIF3f antibody shows detection of endogenous eIF3f in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2). Cdk11 is responsible for phosphorylating eIF3f in vivo. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:200. This antibody recognizes both phosphorylated and non-phosphorylated eIF3f. Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ.


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