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Home Antibody All anti-EIF3F antibodies

Anti-EIF3F Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319432
  • Rabbit polyclonal anti-EIF3F antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC418450) , 20ug
100ug 325 3-7 Days
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WB(1)
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Also for EIF3F (NM_003754)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 114-125 of human eIF3f protein.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat, chimpanzee, chicken, bovine Concentration1.0 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:10,000 - 1:40,000, WB: 1:200 - 1:2,000, IHC: User Optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note eIF3f, also known as eukaryotic translation initiation factor 3 subunit 5, eIF-3 epsilon, and eIF3 p47 subunit, binds to the 40S ribosome and promotes the binding of methionyl-tRNA and mRNA. EIF3f also associates with the complex p170-eIF3. eIF-3 is composed of at least 12 different subunits, eIF3f is one of these subunits.

Reference Data

Target NameHomo sapiens eukaryotic translation initiation factor 3, subunit F (EIF3F)
Alternative NameeIF3-p47; EIF3S5
Database LinkNP_003745
Function
Related Pathway

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WB Image
Western blot using affinity purified anti-eIF3f antibody shows detection of endogenous eIF3f in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2). Cdk11 is responsible for phosphorylating eIF3f in vivo. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:200. This antibody recognizes both phosphorylated and non-phosphorylated eIF3f. Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ.

 

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