OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-HTT antibodies

Anti-HTT PHOSPHO Antibody

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319425
  • Rabbit polyclonal Huntingtin phospho S421 antibody (Phospho-specific)
100ug 325 3-7 Days
Add to Shopping Cart
WB(2)
IHC(1)

OriGene Data

ImmunogenHuntingtin pS421 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 416-424 of Human huntingtin protein.
Clone Name IsotypeIgG
Species Reactivityhuman Concentration0.6 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:10,000 - 1:40,000, WB: 1:500 - 1:3,000, IHC: 1:50 - 1:100
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Huntingtin (also known as Huntington's disease protein, Htt and HD protein) is the protein product of a disease gene linked to Huntington's disease, a neuro-degenerative disorder characterized by loss of striatal neurons. This may be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product (see partial protein sequence below). The huntingtin gene locus is large, spanning 180 kb and consisting of 67 exons.  It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. Normal huntingtin protein shows a cytoplasmic localization.  This protein is widely expressed with the highest level of expression in the brain (nerve fibers, varicosities, and nerve endings). In the brain, the regions where it can be mainly found are the cerebellar cortex, the neocortex, the striatum, and the hippocampal formation.

Reference Data

Target NameHomo sapiens huntingtin (HTT)
Alternative NameHD; IT15
Database LinkNP_002102
Function
Related Pathway
EGFR1 Signaling Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis is shown using Affinity Purified anti-Huntingtin pS421 antibody to detect endogenous protein present in an unstimulated human PC-3 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~190 kDa corresponding to truncated human Huntingtin protein.  The blot was incubated with a 1:1,000 dilution of the antibody at room temperature followed by detection using standard techniques.  Personal communication,
WB Image
Western blot analysis after AKT and phosphatase treatment is shown using Affinity Purified anti-Huntingtin pS421 antibody. In A) untreated lysates from N2A and 293A cells were stained directly using anti-Huntingtin pS421 antibody. Full length staining of Huntingtin is noted, albeit at low levels of expression, as well as a strongly staining band at 200 kDa that may represent staining of truncated protein. In B) staining is shown after immunoprecipitation using a monoclonal antibody (Mab2166) followed by AKT treatment (to phosphorylate), along with untreated, and phosphatase (PP) treated (dephosphorylate) immunopreci-pitated Htt. Full length phosphorylated huntingtin is clearly detected in these immunopurified samples (except dephosphorylated). In C) lysates are treated directly with AKT or PP to alter the phosphorylation status of Htt. Personal communication, Simon Warby, CMMT, Vancouver, BC.
IHC Image
Affinity Purified anti-Huntingtin pS421 antibody was used at a 1:100 dilution to detect phosphorylated Huntingtin by immunohistochemistry in human brain cerebellum.  Positive cytoplasmic staining is observed in neurons. Tissue was formalin-fixed and paraffin embedded.  Personal Communication,

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title