OriGene Logo
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
Home Antibody All anti-ASAP1 antibodies

Anti-ASAP1 PHOSPHO Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA319422 Rabbit polyclonal ASAP1 phospho Y782 antibody (Phospho-specific) 100ug $356 3-7 Days
Add to Shopping Cart

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 777-787 of mouse ASAP1 protein.
Clone Name IsotypeIgG
Species Reactivitymouse, human, chicken, bovine, dog, rat, chimpanzee Concentration1.09 mg/mL
Guaranteed Application *IHC, IF Suggested DilutionsELISA: 1:4,000 - 1:16,000, WB: 1:500 - 1:2,000, IHC: 20-40 ug/ml
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. ASAP1 (also known as AMAP1, 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1 GTPase-activating protein, PIP2-dependent ARF1 GAP,ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, Development and differentiation-enhancing factor 1, Differentiation-enhancing factor 1, DEF-1) is an Arf-directed GTPase activating protein that is a substrate for the kinases Src and FAK and has been implicated in the regulation of membrane traffic, focal adhesions and invadopodia/podosomes.  Phosphorylation of ASAP1 at tyrosine 782 has been found to affect enzymatic and some biological activities, including the function of invadopodia. ASAP1 is expressed in many tissues but is most abundant in the testis, brain, lung and spleen. A heightened expression was seen in the adipose tissue from obese (ob) and diabetic (db) animals. Multiple transcript variants have been reported for this protein.

Reference Data

Target NameMus musculus ArfGAP with SH3 domain, ankyrin repeat and PH domain1 (Asap1), transcript variant 1
Alternative NameAV239055; Ddef1; DEF-1; mKIAA1249; PAP; s19
Database LinkNP_034156
Entrez Gene 50807 Human
Entrez Gene 13196 Mouse
Entrez Gene 314961 Rat
Entrez Gene 482044 Dog
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

IHC Image
Anti-ASAP1 pY782 antibody was used at 20 ?g/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate intracellular positive staining in epidermal keratinocytes in human skin at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communi-cation, Tina Roush, LifeSpanBiosciences, Seattle, WA.

IF Image
Immunofluorescent microscopy using Affinity Purified anti-ASAP1 pY782 antibody shows detection of phosphorylated ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells. Personal Communication. Paul Randazzo, NIH, CCR, Bethesda, MD.


Inc 5000 Healthcare Company

Notice to Non-US Customers

ExclaimationWeb price and delivery time are for direct sales only.

Non-US customers are encouraged to contact our dedicated distributor network for quotes and streamlined ordering/delivery support.