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Home Antibody All anti-RAD23B antibodies

Anti-RAD23B Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319410
  • Goat polyclonal anti-HR23B antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401012) , 20ug Explanation
100ug 325 3-7 Days
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WB(1)
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Also for RAD23B (NM_002874)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 163-176 of human HR23B protein.
Clone Name Isotype
Species Reactivityhuman Concentration1.1 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note HR23B (also known as UV excision repair protein RAD23 homolog B, XP-C repair complementing complex 58 kDa protein and p58) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Reference Data

Target NameHomo sapiens RAD23 homolog B (S. cerevisiae) (RAD23B), transcript variant 1
Alternative NameHHR23B; HR23B; P58
Database LinkNP_002865
Entrez Gene 5887 Human
Function
Related PathwayDruggable Genome Nucleotide excision repair

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using affinity purified anti-HR23B antibody shows detection of a band at ~58 kDa (arrowhead) corresponding to HR23B present in a HeLa whole cell lysate.  Pre-incubation of antibody with immunizing peptide completely blocks reactivity (data not shown).   Approximately 33 µg of lysate was separated by 4-20% Tris Glycine SDS-PAGE. After blocking the membrane was probed overnight at 4°C with the primary antibody diluted to 1:500 in 5% BLOTTO in PBS. The membrane was washed and reacted with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] (605-432-013) for 45 min at room temperature (800 nm channel, green).  Molecular weight estimation was made by comparison to prestained MW markers indicated at left (700 nm channel, red).  IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.

 

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