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Anti-STAT5A PHOSPHO Antibody
Also for STAT5A (NM_011488)
|This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Y694 of mouse STAT5a protein.|
|human, mouse, rat
||ELISA: 1:25,000, WB: 1:500 - 1:2,000, IHC: 20-40 ug/ml
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Signal trans-ducer and activator of transcription 5 (Stat5) belongs to a family of cytoplasmic trans-cription factors that can be activated (phosphorylated) by a cell surface receptor. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Stat5 has two isoforms, Stat5a and Stat5b. Aberrant Stat5 activation has been implicated in the pathogenesis of chronic myelogenous leukemia, prostate and breast cancer and tumor metastasis. Stat5 is localized in the cytoplasm and upon phosphorylation at Y694 is translocated to the nucleus.
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Western blot using affinity purified anti-Stat5 pY694 antibody shows detection of phosphorylated Stat5 (indicated by arrowhead at ~91 kDa) in NK92 cells after 30 min treatment with 1Ku of IL-2 (lane 2). No reactivity is seen for non-phosphorylated Stat5 in untreated cells (lane 1). The membrane was probed with the primary antibody at a 1:1,000 dilution, overnight at 4° C. Personal Communication from Ana Gamero, CCR-NCI, Bethesda, MD.
affinity purified anti-Stat5 pY694 antibody was used at 20g/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate positive staining of distal convoluted tubules and collecting ducts of human kidney at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. Personal Communication, Tina Roush, LifeSpanBiosciences, Seattle, WA.