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Anti-LDB1 Antibody


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SKU Description Amount Price Availability*  
TA319355 Rabbit polyclonal anti-LDB1 antibody 100ug $325 3-7 Days

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OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 361-373 of mouse LDB1 protein.
Clone Name IsotypeIgG
Species Reactivitymouse, human, rat Concentration1.80 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:425,000, WB: 1:500 - 1:3,000, ChIP: User Optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note LDB1 is also known as CLIM 2, LIM Domain Binding 1, NLI and Nuclear LIM Domain Interactor. The LIM-domain binding protein binds to the LIM domain of LIM homeodomain proteins which are transcriptional regulators of development. Nuclear LIM interactor (NLI) / LIM domain-binding protein 1 (LDB1) is located in the nuclei of neuronal cells during development, it is co-expressed with Isl1 in early motor neuron differentiation and has a suggested role in the Isl1 dependent development of motor neurons. It is suggested that these proteins act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors, both of which have essential roles in development.

Reference Data

Target NameMus musculus LIM domain binding 1 (Ldb1), transcript variant 3
Alternative NameCLIM2; NLI
Database LinkNP_034827
Entrez Gene 8861 Human
Entrez Gene 16825 Mouse
Entrez Gene 309447 Rat
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot using affinity purified anti-LDB1 antibody shows detection of LDB1 protein (arrowhead) in Jurkat whole cell lysate.   Approximately 30 µg of lysate was loaded prior to separation and transfer to nitrocellulose.  Primary antibody was used at a 1:1,800 dilution in 5% BLOTTO in PBS reacted overnight at 4°C.  The membrane was washed and reacted with a 1:20,000 dilution of DyLight™800 conjugated Gt-a-Rabbit IgG [H&L] MX (611-145-122) for 45 min at room temperature (800 nm channel, green).   Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red).   Fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.


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