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Home Antibody All anti-CLIM1 antibodies

Anti-CLIM1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319341
  • Rabbit polyclonal anti-LDB2 antibody
100ug 325 3-7 Days
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WB(1)
IHC(1)

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 107-120 of Mouse LDB2.
Clone Name IsotypeIgG
Species Reactivitymouse, human, chimpanzee, dog, rat, chicken Concentration1.06 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note LDB2 (LIM homeobox protein cofactor CLIM-1a).  It is suggested that LIM domain binding proteins (LDB1, LDB2 and LDB3) act synergistically to enhance transcriptional efficiency by acting as co-factors for LIM homeodomain and Otx class transcription factors both of which have essential roles in development. LDB2 is a close homologue of LDB1.  LDBs are homologs of the Drosophila Chip protein. Three members of the ldb gene family (Ldb1, Ldb2 and Ldb3) from the zebrafish, Danio rerio, share 95%, 73% and 62% amino acid identity with mouse Ldb1, respectively.

Reference Data

Target NameMus musculus LIM domain binding 2 (Ldb2), transcript variant 1
Alternative NameAI035351; AW146358; CLIM1; CLP-36; Ldb3
Database LinkNP_034828
Function
Related Pathway

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WB Image
Western blot using Affinity Purified anti-LDB2 antibody shows detection of a 43-kDa band corresponding to LDB2 in a lysates prepared from human kidney (lane 1) and mouse spleen (lane 2) tissues.  Approximately 18 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-LDB2 antibody.  Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature.  A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.
IHC Image
Affinity Purified anti-LDB2 (Clim1) antibody was used at a 5 µg/ml to detect LDB2 in human brain cortex tissue. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain. Tissue was formalin-fixed and paraffin embedded.

 

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