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Anti-FBXO43 Antibody


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  • Rabbit polyclonal anti-Fbp5B antibody
100ug $325 3-7 Days
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OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region approximately 60 residues downstream of the amino terminal end of human Fbp5B protein. Human Fbp5B-2, 466 aa, predicted MW 51.3 kDa
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat, chimpanzee Concentration2.21 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:10,000 - 1:50,000, WB: 1:500 - 1:3,000, IP: 1:100
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Fbp5B (also called F-Box protein 43 or Fbx043) is a relatively new member of the F-box protein family that is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class.

Reference Data

Target NameHomo sapiens F-box protein 43 (FBXO43), transcript variant 2
Alternative NameEMI2; ERP1; FBX43
Database LinkNP_001025031
Entrez Gene 286151 Human
Entrez Gene 78803 Mouse
Entrez Gene 315034 Rat
Related Pathway Oocyte meiosis

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* OriGene provides validated application data and protocol, with money back guarantee.

Western blot using affinity purified anti-Fbp5B antibody. Panels A and B show two identical membranes containing marker, in vitro translated Fbp5b (Fbp5b ivt), and a whole cell extract from 293T cells loaded in duplicate). Panel A shows antibody reactivity. Panel B shows antibody reactivity after first pre-incubating the antibody with the immunizing peptide. This pre-incubation greatly diminishes specific band recognition. Peptide inhibition completely removes the in vitro translated band and greatly reduces the endogenous band from 293T cells. When this experiment was repeated using a mock in vitro translation, no band was detected (data not shown). Personal Communication, Daniele Guardavaccaro, NYU Cancer Institute


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