Home Antibody All anti-A2M antibodies
|a2-Macroglobulin [Human Plasma]|
||ELISA: 1:20,000 - 1:100,000, WB: 1:2,000 - 1:10,000, IHC: 1:500 - 1:2,500
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Homo sapiens alpha-2-macroglobulin (A2M)|
|A2MD; CPAMD5; FWP007; S863-7|
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Goat anti Alpha-2-Macroglobulin (200-101-207 lot 20172) was used to detect Alpha-2-Macroglobulin under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:3000 dilution of primary antibody (ON 4 C in MB-070). Detection shown was using Dylight 649 conjugated Donkey anti goat (605-743-125 lot 20834 1:10K in TBS/MB-070) 1 hr RT. Images were collected using the BioRad VersaDoc System.
Western Blot of Goat anti-Alpha-2-Macroglobulin Antibody. Lane 1: Alpha-2-Macroglobulin. Lane 2: none. Load: 100 ng per lane. Primary antibody: Alpha-2-Macroglobulin antibody at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 800 goat secondary antibody at 1:20,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 163 kDa, 178 kDa for Alpha-2-Macroglobulin. Other band(s): Alpha-2-Macroglobulin splice variants and isoforms.