Home Antibody All anti-AKT1 antibodies
Also for AKT1 (NM_001014431)
|Anti-AKT antibody was affinity purified antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human AKT1 protein. A residue of cysteine was added to facilitate coupling.|
||ELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000, IHC: user optimized, IF: user optimized
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
|Homo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 3|
|AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA|
Entrez Gene 207 Human
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
MAPK signaling pathway
Signaling by GPCR
Toll-like receptor signaling pathway
Wnt Signaling Pathway
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Western Blot of Rabbit Anti-AKT Antibody. Lane 1: HeLa cells. Lane 2: H2O2 treated MCF7 cells. Lane 3: Recombinant AKT. Lane 4: A431 cells. Load: 35 µg per lane. Primary antibody: AKT antibody at 1:300 for overnight at 4°C. Secondary antibody: IRDYE800™ conjugated Dnky-a-Sheep IgG was used at a 1:10,000 dilution for 45 min at room temperature. Block: 1% BLOTTO overnight at 4°C. Predicted/Observed size: ~55-60 kDa for AKT . Other band(s): unspecific.