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Home Antibody All anti-AKT1 antibodies

Anti-AKT1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319277
  • Rabbit polyclonal anti-AKT antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC423056) , 20ug
200ul 325 3-7 Days
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WB(2)
IHC(1)
IF(1)
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Also for AKT1 (NM_001014431)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenAKT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human, rat and mouse and chicken AKT proteins conjugated to KLH using maleimide.  A residue of cysteine was added to the amino terminal end to facilitate coupling.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat, chicken Concentration75 mg/mL
Guaranteed Application *WB, IHC, IF Suggested DilutionsELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,000, IHC: 1:500 - 1:2,000, IF: 1:100 - 1:1,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note AKT Antibody detects AKT which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308.  AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways.  AKT Exhibits tight control over cell proliferation and cell viability.  Overexpression or inappropriate activation of AKT is noted in many types of cancer.   AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.  AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.

Reference Data

Target NameHomo sapiens v-akt murine thymoma viral oncogene homolog 1 (AKT1), transcript variant 3
Alternative NameAKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
Database LinkNP_001014431
Function
Related Pathway
Apoptosis
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
Focal Adhesion
Hemostasis
Jak-STAT signaling pathway
MAPK signaling pathway
Signaling by GPCR
Toll-like receptor signaling pathway
Wnt Signaling Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western Blot of simultaneous detection of unphosphorylated and phosphorylated Rabbit Anti-AKT antibody. Lane 1: unstimulated NIH/3T3 lysates contain inactive unphosphorylated Akt1, green band. Lane 2: PDGF stimulated NIH/3T3 lysate contains both inactive (green band) and activated phosphorylated Akt1 (red band). Load: 35 µg per lane. Primary antibody: rabbit anti-Akt (pan) and mouse anti-Akt pS473 specific antibodies at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 549 conjugated anti-rabbit IgG (green) and DyLight™ 649 conjugated anti-mouse IgG (red) secondary antibodies at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.
WB Image
Western Blot of Rabbit Anti-AKT antibody. Lane 1: NIH/3T3 whole cell lysate. Load: 20 ug per lane. Primary antibody: AKT antibody at 1:500 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Rabbit IgG (611-103-122) at 1:10,000 preceded color development using Pierce Chemical's SuperSignal™ substrate. Block: MOPS buffer overnight at 4°C. Predicted/Observed size: 128 kDa, 128 kDa for AKT. Other band(s): none.
IHC Image
Immunohistochemistry of Rabbit Anti-AKT antibody. Tissue: (A) normal colon tissue, (B) colon tumor tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT antibody at 1:1,000 dilution for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AKT is nuclear. Staining: AKT as precipitated red signal with hematoxylin purple nuclear counterstain.
IF Image
Immunofluorescence Microscopy of Rabbit Anti-AKT Antibody. Tissue: neonatal rat cardiomyocytes. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AKT antibody at 1:80 dilution for 1 h at RT. Secondary antibody: Texas-red™ conjugated rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AKT is nuclear. Staining: Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red™ conjugated phalloidin.

 

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