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Anti-NEK2 Antibody


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SKU Description Amount Price Availability*  
  • Rabbit polyclonal anti-NEK2 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419288) , 20ug Explanation
100ug $325 3-7 Days
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OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 287-299 of Human NEK2 protein.
Clone Name IsotypeIgG
Species Reactivityhuman Concentration1.0 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,000, IF: User Optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note NEK2, also called serine/threonine-protein kinase Nek2NimA-related protein kinase 2, NimA-like protein kinase 1 and HSPK 21, is a protein kinase involved in mitotic regulation that may have a role at the G2-M transition and may also play a role in meiosis. NEK2 has a nuclear localization and accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins.

Reference Data

Target NameHomo sapiens NIMA-related kinase 2 (NEK2), transcript variant 3
Alternative NameHsPK21; NEK2A; NLK1; PPP1R111; RP67
Database LinkNP_001191111
Entrez Gene 4751 Human
Related PathwayProtein KinaseDruggable Genome

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WB Image
Western blot analysis is shown using Affinity Purified anti-NEK2 antibody to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein.  The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.  Personal communication Steven Pelech, Kinexus Inc.


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