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Home Antibody All anti-UBC13 antibodies

Anti-UBC13 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319258
  • Goat polyclonal anti-UBC13 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC418741) , 20ug Explanation
100ug 325 3-7 Days
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WB(1)
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Also for UBC13 (NM_003348)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 40-51 of human UBC13 protein.
Clone Name Isotype
Species Reactivityhuman Concentration1.25 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:2,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note UBC13 is a member of the E2 ubiquitin-conjugating enzyme family also known as Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N, Ubiquitin carrier protein N and Bendless-like ubiquitin-conjugating enzyme.  The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene

Reference Data

Target NameHomo sapiens ubiquitin-conjugating enzyme E2N (UBE2N)
Alternative NameHEL-S-71; UBC13; UbcH-ben; UbcH13; UBCHBEN
Database LinkNP_003339
Entrez Gene 7334 Human
Function
Related PathwayDruggable Genome Ubiquitin mediated proteolysis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using affinity purified anti-UBC13 antibody shows detection of UBC13 protein in human small intestine lysate (lane 1) but not in mouse thymus lysate (lane 2). The heavily stained band in lane 1 (arrowhead) indicates this particular gel was overloaded with protein. The identity of minor reactive bands is unknown but could represent E2 complexes. Each lane contains approximately 20 ug of lysate. Primary antibody was used at a 1:500 dilution. The membrane was washed and reacted with a 1:10,000 dilution of Alexa FluorTM 680 conjugated Rb-a-Goat IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.

 

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