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Home Antibody All anti-NFKB1 antibodies

Anti-NFKB1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319254
  • Rabbit polyclonal anti-NFKB p50 antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC418264) , 20ug
100ul $325 3-7 Days
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WB(1)

OriGene Data

ImmunogenHuman NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Clone Name IsotypeIgG
Species Reactivityhuman Concentration80 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:2,000
BufferNone
Note Anti NFkB p50 Antibody recognizes NFKB p50 which is a component of NFKB. NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells.  It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkB bound to IkB. NFkB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkB-a. IkB-a binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.

Reference Data

Target NameHomo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1), transcript variant 2
Alternative NameEBP-1; KBF1; NF-kappa-B; NF-kappaB; NF-kB1; NFkappaB; NFKB-p105; NFKB-p50; p105; p50
Database LinkNP_001158884
Function
Related Pathway
Apoptosis
MAPK signaling pathway
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway

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WB Image
All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.  The membrane was blocked in 5% dry milk for 2 h.  After washing, a:1:1,000 dilution of the primary antibody was added to the membrane and incubated for 2 h.  Washes with buffer were performed 4 times for 5' each.  The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1 h.   Washes with TBS preceded color development.

 

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