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Home Antibody All anti-SMAD1 antibodies

Anti-SMAD1 Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319239
  • Rabbit polyclonal anti-SMAD1 antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC416996) , 20ug
100ug 325 3-7 Days
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WB(1)
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Also for SMAD1 (NM_001003688)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 206 of human SMAD1 protein.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat, dog Concentration1.31 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:25,000-1:30,000, WB: 1:500, IP: 2 ug
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note SMAD1 is also known Mothers Against Decapentaplegic homolog 1, Mothers against DPP homolog 1, hSMAD-3, JV4-1, Transforming growth factor-Beta-Signaling Protein 1 or BSP1. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1, as a transcriptional modulator, is activated by BMP (Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD or R-SMAD). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD proteins have been implicated as downstream effectors of TGF beta/BMP signaling. In response to BMP ligands, SMAD1 can be phosphorylated (other sites besides the most prominent of S206, are S187, S195, and S214). S-206 is phosphorylated by ERK in response to mitogenic growth factors, or by recombinant ERK in vitro; this can be tested by treating cells with EGF or in cancer cells where Ras is activated. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is also a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.

Reference Data

Target NameHomo sapiens SMAD family member 1 (SMAD1), transcript variant 2
Alternative NameBSP-1; BSP1; JV4-1; JV41; MADH1; MADR1
Database LinkNP_001003688
Function
Related Pathway
Delta-Notch Signaling Pathway
TGF Beta Signaling Pathway

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WB Image
Western blot using Affinity Purified anti-SMAD1 antibody shows detection of endogenous SMAD1 in whole cell lysates from human hepatoma (HEPG2, lanes 1-4) and keratinocyte (HaCaT, lanes 5-8) derived cell lines treated with PBS, BMP2, EGF, or NaCl for 1 h at 37°C before harvest. Each lane contains approximately 15 µg of lysate. Primary antibody was used at a 1:500 dilution in 1% BLOTTO (p/n B501-0500) and reacted for 1 hour at room temperature. Anti-beta actin (p/n 600-401-886) staining was used as a loading control. The membrane was washed and reacted with a 1:3,000 dilution HRP-conjugated a-Rabbit IgG (p/n 611-103-122) for 1 hour at room temperature. Detection was by ECL. Personal communication, Xin-Hua Feng, Baylor College of Medicine, Houston, TX

 

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