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Home Antibody All anti-CCNB1 antibodies

Anti-CCNB1 Antibody

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SKU Description Amount Price Availability*  
TA319229
  • Rabbit polyclonal Cyclin B1 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC410417) , 20ug Explanation
100ul 325 3-7 Days
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WB(2)
IHC(1)

OriGene Data

ImmunogenAnti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.
Clone Name IsotypeIgG
Species Reactivityhuman, rat, mouse Concentration80 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:1,000, IP: 1:100
BufferNone
Note The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis using anti-Cyclin B1 antibody shows detection of Cyclin B1 present in asynchronous HeLa cell lysates. Comparison to a molecular weight marker indicates a band of ~55 kDa corresponding to human Cyclin B1 (arrowhead). Approximately 50 µg of lysate was loaded on to a 7% SDS-PAGE gel for separation. After transfer to nitrocellulose, the blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG (p/n 611-103-122). Personal communication, Luca D'Agostino, Temple University, Philadelphia, PA.
WB Image
Western blot analysis using anti-cyclin B1 antibody shows detection of human Cyclin B1 present in asynchronous HN30 cell lysates.  HN30 cells are from head and neck cancer tumors that over express cyclin B1 and D1. Comparison to a molecular weight marker indicates a band of ~62 kDa corresponding to the expected molecular weight for the protein (arrowhead).  The blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature.  Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG 611-103-122 and chemiluminescence reagent with a 1-min exposure time.  Other detection systems will yield similar results. Personal communication, Luca Cote, Temple University, Philadelphia, PA.
IHC Image
anti-Cyclin B1 antibody was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

 

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