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Home Antibody All anti-CCNB1 antibodies

Anti-CCNB1 Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA319229 Rabbit polyclonal Cyclin B1 antibody 100ul $325 3-7 Days
LC410417 CCNB1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(2)
IHC(1)

OriGene Data

ImmunogenAnti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.
Clone Name IsotypeIgG
Species Reactivityhuman, rat, mouse Concentration80 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:1,000, IP: 1:100
BufferNone
Note The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB analysis using anti-Cyclin B1 antibody shows detection of Cyclin B1 present in asynchronous HeLa cell lysates. Comparison to a molecular weight marker indicates a band of ~55 kDa corresponding to human Cyclin B1 (arrowhead). The blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG.
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WB analysis using anti-cyclin B antibody shows detection of human Cyclin B present in asynchronous HN30 cell lysates.HN30 cells are from head and neck cancer tumors that over express cyclin B and D. Comparison to a molecular weight marker indicates a band of ~62 kDa corresponding to the expected molecular weight for the protein (arrowhead).  The blot was incubated with a 1:500 dilution of the antibody.Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG.
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anti-Cyclin B1 antibody was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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