Home Antibody All anti-CCNB1 antibodies
Also for CCNB1 (NM_031966)
|Anti-Cyclin B1 antibody was produced by repeated immunizations of full length fusion protein corresponding to the human gene.|
|human, rat, mouse
||ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:1,000, IP: 1:100
|The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
|Homo sapiens cyclin B1 (CCNB1)|
Hedgehog signaling pathway
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Western blot analysis using anti-Cyclin B1 antibody shows detection of Cyclin B1 present in asynchronous HeLa cell lysates. Comparison to a molecular weight marker indicates a band of ~55 kDa corresponding to human Cyclin B1 (arrowhead). Approximately 50 µg of lysate was loaded on to a 7% SDS-PAGE gel for separation. After transfer to nitrocellulose, the blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG (p/n 611-103-122). Personal communication, Luca D'Agostino, Temple University, Philadelphia, PA.
Western blot analysis using anti-cyclin B<sub>1</sub> antibody shows detection of human Cyclin B<sub>1</sub> present in asynchronous HN30 cell lysates. HN30 cells are from head and neck cancer tumors that over express cyclin B<sub>1</sub> and D<sub>1</sub>. Comparison to a molecular weight marker indicates a band of ~62 kDa corresponding to the expected molecular weight for the protein (arrowhead). The blot was incubated with a 1:500 dilution of the antibody for 1 h at room temperature. Detection occurred using a 1:10,000 of HRP conjugated Goat-a-Rabbit IgG 611-103-122 and chemiluminescence reagent with a 1-min exposure time. Other detection systems will yield similar results. Personal communication, Luca Cote, Temple University, Philadelphia, PA.
anti-Cyclin B1 antibody was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.