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Anti-ING5 Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA319227 Goat polyclonal anti-p28 ING5 antibody 100ug $356 3-7 Days
LC410205 ING5 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 127-140 of Human p28 ING5protein (Inhibitor of growth family, member 5). This sequence shows homology to isoforms 1 and 2 for ING5.
Clone Name Isotype
Species Reactivityhuman Concentration1.52 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:4,000 - 1:16,000, WB: 1:500 - 1:1,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note p28 ING5 is a tumor suppressor protein similar to ING1 that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway.  Multiple alternatively spliced transcript variants have been observed.  The accession number listed below is for variant (1) encodes the longest isoform.

Reference Data

Target NameHomo sapiens inhibitor of growth family member 5 (ING5), transcript variant 1
Alternative Namep28ING5; p28ING5
Database LinkNP_115705
Entrez Gene 84289 Human
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
WB analysis using Anti-p28 ING5 antibody to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This WB shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification in lane 1. The blot was incubated with a 1:500 dilution of the antibody at RT.

WB Image
WB analysis using Anti-p28 ING5 antibody to detect over expressed Human ING5 present in cell extracts. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kDa corresponding to the expected molecular weight for the recombinant protein. Approximately 10 µg of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500.


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