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Also for IDO2 (NM_194294)
|IDO2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a mixture of human and mouse full length protein corresponding to IDO2.|
||ELISA: 1:500-1:100,000, WB: 1:2,000-1:10,000, IF: 1:50-1:1000, IP: 1:50-1:1000
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|IDO2 antibody recognizes Indoleamine 2,3-dioxygenase 2 (IDO2). IDO2 is related to IDO1, and may play a role in T and B cell immunity. IDO2 is expressed in a few select cell types and levels of protein expression appear to be very low, perhaps below the level of detection by IHC. It is detected in liver, small intestine, spleen, placenta, thymus, lung, brain, kidney, and colon. IDO2 catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. Activity of IDO2 is inhibited by 1MT (1-methyl-tryptophan) and MTH-trp (methylthiohydantoin-DL-tryptophan). D-1mT (1-methyl-D-tryptophan) may be an indirect inhibitor of the IDO2 pathway. Small-molecule inhibitors of indoleamine 2,3-dioxygenase (IDO) are currently being translated to clinic for evaluation as cancer therapeutics.
|Homo sapiens indoleamine 2,3-dioxygenase 2 (IDO2)|
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Western Blot of Anti-IDO2 Antibody. Left Figure: Lane 1: HEK293 expressing human IDO-1 V5 tag. Lane 2: HEK293 expressing human IDO-2 V5 tag. Primary antibody: V5 antibody. Secondary antibody: rabbit secondary antibody HRP. Right Figure: Lane 1: HEK293 expressing human IDO-1 V5 tag. Lane 2: HEK293 expressing human IDO-2 V5 tag. Primary antibody: IDO-2 antibody at 1:2000-10,000. Secondary antibody: rabbit secondary antibody HRP. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 45.4 kDa, 45 kDa for IDO2. Other band(s): unknown.
Immunofluorescence of Anti-IDO2 Antibody. Tissue: human IDO2 expressing HEK293 cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary: IDO2 antibody at 1:50-1:1000 for 1 h at RT. Secondary: Peroxidase rabbit secondary antibody at 1:10,000 for 45min at RT. Localization: IDO-2 is located in the cytosol. Staining: IDO-2 as green fluorescent signal with bis-benzimide nuclear counterstain (blue).