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Anti-HIF1A Antibody


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SKU Description Amount Price Availability*  
  • Rabbit polyclonal Hif-1 alpha hydroxy P564 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403613) , 20ug Explanation
100ul $325 In Stock
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OriGene Data

ImmunogenThis antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region surrounding the P564 of human HIF-1a.
Clone Name IsotypeIgG
Species Reactivityhuman, monkey, mouse, rat, dog, bovine, Xenopus Concentration85 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:5,000 - 1:20,000, WB: 1:500 - 1:2,000, IHC: User Optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Tumor hypoxia often directly correlates with aggressive phenotype, metastasis progression and resistance to chemotherapy. HIF-1 transcription factors are dramatically induced in hypoxic areas and regulate the expression of genes necessary for tumor adaptation to conditions of low oxygen. The stabilization of HIF-1a by hypoxia is critically dependent upon the hydroxylation of certain Proline residues that exist in the oxygen-dependent degradation domain of HIF-1a. HIF factors are now considered an important therapeutic target for cancer intervention.

Reference Data

Target NameHomo sapiens hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant 3
Alternative NamebHLHe78; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
Database LinkNP_001230013
Entrez Gene 3091 Human
Entrez Gene 15251 Mouse
Entrez Gene 29560 Rat
Entrez Gene 704678 Monkey
Entrez Gene 480348 Dog
Related PathwayTranscription Factors mTOR signaling pathwayPathways in cancerRenal cell carcinoma

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WB Image
Western blot using anti-HIF-1alphaHYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kDa in size corresponding to HIF1-????The primary antibody was used at a 1:1,000 dilution in 2% BLOTTO. Personal Communication, L. Neckers and O. Aprelikova, NCI, Bethesda, MD.


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