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Home Antibody All anti-RELA antibodies

Anti-RELA Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319206
  • Rabbit polyclonal NFkB p65 (RelA) Phospho S529 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402895) , 20ug Explanation
100ul 325 3-7 Days
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WB(3)
IHC(1)

OriGene Data

ImmunogenNFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 529 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Clone Name IsotypeIgG
Species Reactivityhuman Concentration75 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000, IHC: 1:200 - 1:1,000
BufferNone
Note NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. Anti-NFkB antibody is ideal for Cell Biology, Nuclear Signaling, Neuroscience and Signal Transduction Research

Reference Data

Target NameHomo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), transcript variant 2
Alternative NameNFKB3; p65
Database LinkNP_001138610
Entrez Gene 5970 Human
Function
Related PathwayTranscription FactorsDruggable Genome MAPK signaling pathwayChemokine signaling pathwayApoptosisToll-like receptor signaling pathwayNOD-like receptor signaling pathwayRIG-I-like receptor signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method. Other detection methods will yield similar results. Data contributed by Aggarwal BB, personal communication.
WB Image
Anti-pS529 shows phospho p65 staining in carcinoma cells.
WB Image
TNF Induces phosphorylation of p65 in KBM-5 cells.  Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65.  Immunochemical's pan reactive anti p65 (code# 100-4165) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts.  Phosphorylation of p65 occurs after approximately 10 min of TNF exposure.  Migration of phosphorylated p65 into the nucleus occurs within a similar time frame.   HRP conjugated Gt-anti-Rabbit IgG was used to develop the western blot using a chemi-luminescent detection method.  Other detection methods will yield similar results. Personal Communication, Aggarwal BB.
IHC Image
Anti-pS276 shows phospho p65 staining in carcinoma cells.

 

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