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Home Antibody All anti-RELA antibodies

Anti-RELA Antibody

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA319206 Rabbit polyclonal NFkB p65 (RelA) Phospho S529 antibody 100ul $325 3-7 Days
LC402895 RELA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(3)
IHC(1)

OriGene Data

ImmunogenNFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 529 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Clone Name IsotypeIgG
Species Reactivityhuman Concentration75 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:3,000, IHC: 1:200 - 1:1,000
BufferNone
Note NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. Anti-NFkB antibody is ideal for Cell Biology, Nuclear Signaling, Neuroscience and Signal Transduction Research

Reference Data

Target NameHomo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), transcript variant 2
Alternative NameNFKB3; p65
Database LinkNP_001138610
Entrez Gene 5970 Human
Function
Related PathwayTranscription FactorsDruggable Genome MAPK signaling pathwayChemokine signaling pathwayApoptosisToll-like receptor signaling pathwayNOD-like receptor signaling pathwayRIG-I-like receptor signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

TNF Induces phosphorylation of p65 in KBM-5 cells. Nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS529 p65. Anti-beta-actin staining confirms loading of equivalent amounts of protein. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method. Data contributed by Aggarwal BB, personal communication.
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Anti-pS529 shows phospho p65 staining in carcinoma cells.
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TNF Induces phosphorylation of p65 in KBM-5 cells.  Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65.  Immunochemical's pan reactive anti p65was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts.  Phosphorylation of p65 occurs after approximately 10 min of TNF exposure.
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Anti-pS276 shows phospho p65 staining in carcinoma cells.
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