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Anti-RELA Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319203
  • Rabbit polyclonal NFkB p65 (RelA) Phospho S276 antibody
  • FREE positive control: HEK293T cell transient overexpression lysate (LC402895) , 20ug
100ul 325 3-7 Days
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WB(4)
IHC(1)

OriGene Data

ImmunogenNFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).  Sequence information:  QLRRPpSDRELSC
Clone Name IsotypeIgG
Species Reactivityhuman Concentration80 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:10,000 - 1:30,000, WB: 1:1,000 - 1:5,000, IHC: 1:200-1:800
BufferNone
Note NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. Anti-NFkB antibody is ideal for Cell Biology, Nuclear Signaling, Neuroscience and Signal Transduction Research

Reference Data

Target NameHomo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), transcript variant 2
Alternative NameNFKB3; p65
Database LinkNP_001138610
Function
Related Pathway
Apoptosis
Delta-Notch Signaling Pathway
MAPK signaling pathway
Toll-like receptor signaling pathway

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WB Image
Anti-pS529 shows phospho p65 staining in carcinoma cells.
WB Image
Anti-pS276 shows phospho p65 staining in carcinoma cells. Western blot of total protein lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS276 p65. Lanes 1-6 contain protein lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes and does not show significant levels of phosphorylated p65. Lane 8 contains protein lysate from ATCC SCC9 cells (also a head and neck squamal carcinoma). Lane 9 contains lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard. Concurrent staining with anti-beta microtubulin (not shown) was used to confirm equal protein loading in all lanes. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemiluminescent detection method. Other detection methods will yield similar results. Data contributed by Yu, M., NIH, personal communication.
WB Image
TNF Induces phosphorylation of p65 in KBM-5 cells.
WB Image
TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Immunochemical's pan reactive anti p65 (code# 100-4165) was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts. Phosphorylation of p65 occurs after approximately 10 min of TNF exposure. Migration of phosphorylated p65 into the nucleus occurs within a similar time frame. HRP conjugated Gt-anti-Rabbit IgG was used to develop the blot using a chemi -luminescent detection method. Other detection methods will yield similar results. Personal Communication, Aggarwal BB
IHC Image
anti-p65 pS276 antibody was diluted 1:500 to detect p65 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

 

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