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Anti-RELA Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA319203 Rabbit polyclonal NFkB p65 (RelA) Phospho S276 antibody 100ul $356 3-7 Days
LC402895 RELA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenNFkB p65 (Rel A) peptide corresponding to a region near phospho Serine 276 of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).  Sequence information:  QLRRPpSDRELSC
Clone Name IsotypeIgG
Species Reactivityhuman Concentration80 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:10,000 - 1:30,000, WB: 1:1,000 - 1:5,000, IHC: 1:200-1:800
Note NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. Anti-NFkB antibody is ideal for Cell Biology, Nuclear Signaling, Neuroscience and Signal Transduction Research

Reference Data

Target NameHomo sapiens v-rel avian reticuloendotheliosis viral oncogene homolog A (RELA), transcript variant 2
Alternative NameNFKB3; p65
Database LinkNP_001138610
Entrez Gene 5970 Human
Related PathwayTranscription FactorsDruggable Genome MAPK signaling pathwayChemokine signaling pathwayApoptosisToll-like receptor signaling pathwayNOD-like receptor signaling pathwayRIG-I-like receptor signaling pathwayMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Anti-pS529 shows phospho p65 staining in carcinoma cells.

WB Image
Anti-pS276 shows phospho p65 staining in carcinoma cells. WB of lysates from various human head and neck tumors shows phospho p65 staining in tumor cell lines using phospho specific polyclonal anti-human pS276 p65. Lanes 1-6 lysates from human squamal carcinoma cell lines. Lane 7 is a protein lysate from a primary culture of human keratinocytes. Lane 8 lysate from ATCC SCC9 cells. Lane 9 lysate from EGF-induced human derived A431 cells. Lane 10 (not shown) contains a molecular weight standard.

WB Image
TNF Induces phosphorylation of p65 in KBM-5 cells.

WB Image
TNF Induces phosphorylation of p65 in KBM-5 cells. Cytoplasmic and nuclear protein lysates prepared after 0, 5, 10, 15, 30 and 60 minutes of 0.1 nM TNF treatment of KBM-5 cells shows inducible phosphorylation using phospho specific polyclonal anti-human pS276 p65. Immunochemical's pan reactive anti p65 was used a control to show the presence of total p65 in both the cytoplasmic and nuclear extracts.

IHC Image
anti-p65 pS276 antibody was diluted 1:500 to detect p65 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.


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