OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-TRAF2 antibodies

Anti-TRAF2 Antibody

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319201
  • Rabbit polyclonal anti-TRAF2 antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402843) , 20ug Explanation
100ug 325 3-7 Days
Add to Shopping Cart
WB(1)
spacer
Also for TRAF2 (NM_021138)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region human TRAF2.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse Concentration0.93 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:60,000 - 1:250,000, WB: 1 ug/mL, IF: user optimized
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note TRAF2, or Tumor Necrosis factor (TNF) Receptor-Associated Factor 2, is an adapter protein and signal transducer that links members of the tumor necrosis factor receptor family to different signaling pathways by association with the receptor cytoplasmic domain and kinases. Association to the receptor is also mediated by the interaction with TRADD. TRAF2 mediates activation of NF-kappa-B and MAPK8/JNK and is involved in apoptosis. TRAF2 forms a heterodimeric complex with TRAF1, which then recruits the inhibitor-of-apoptosis proteins (IAPs), apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2 for the inhibition of caspase activation. In this way it functions as a mediator of the anti-apoptotic signals from TNF receptors. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. TRAF2 may be involved in IL-15 signaling. Multiple alternatively spliced transcript variants exist, but the biological validity of only one transcript has been determined.

Reference Data

Target NameHomo sapiens TNF receptor-associated factor 2 (TRAF2)
Alternative NameMGC:45012; TRAP; TRAP3
Database LinkNP_066961
Entrez Gene 7186 Human
Entrez Gene 22030 Mouse
Function
Related PathwayDruggable Genome MAPK signaling pathwayApoptosisRIG-I-like receptor signaling pathwayAdipocytokine signaling pathwayPathways in cancerSmall cell lung cancerMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using affinity purified anti-TRAF2 antibody shows detection of endogenous TRAF2 in whole HeLa cell lysates. Lane 2 shows endogenous TRAF2 detected with antibody at 47 kDa (arrowhead). Lane 4 shows no reactivity when blot is incubated with immunizing peptide. The identity of lower molecular weight band in lane 2 is unknown. Briefly, each lane contains approximately 14 µg of lysate. Membranes were blocked in 3% BSA-TBS 30 min. at room temperature. Primary antibody was used at a 1:500 dilution in 3% BSA-TBS and reacted overnight at 4°C. The membrane was washed and reacted with a 1:20,000 dilution conjugated Gt-a-Rabbit DyLight 649 (611-143-122) for 1 hr at room temperature. Molecular weight estimation was made by comparison to prestained MW markers in lanes 1 and 3. Fluorescence image was captured using the VersaDoc® Imaging System developed by Bio-Rad.

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title