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Anti-NOTCH1 Antibody


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SKU Description Amount Price Availability*  
TA319200 Rabbit polyclonal anti-NOTCH 1 antibody 200ul $387 3-7 Days
LC402602 NOTCH1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenThis whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat Concentration80 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:20,000 - 1:60,000, WB: 1:500- 1:2,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Anti Notch 1 Antibody recognizes Notch 1 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Anti-Human NOTCH 1 (Cleaved N Terminal) was used at 1:500 to detect mouse Notch 1. Lane M: Marker. Lane 1: No transfection. Lane 2: N1 (mouse deleted extracellular domain)-myc. Lane 3: N1 (mouse intracellular)-myc. Lane 4: N2 (mouse deleted extracellular)-myc. Lane 5: N2 (mouse intracellular)-myc. Lane 6: N3 (mouse deleted extracellular)-myc. Lane 7: N3 (mouse intracellular)-myc. Lane 8: N4 (mouse deleted extracellular)-myc. Lane 9: N4 (mouse intracellular)-myc. Lane 10: N1 (mouse deleted extracellular)(V to G)-myc.


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