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Anti-SMAD3 Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA319188 Rabbit polyclonal anti-SMAD3 antibody 100ug $356 3-7 Days
LC401784 SMAD3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human Smad3 protein.
Clone Name IsotypeIgG
Species Reactivityhuman, mouse, rat Concentration1.06 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:15,000 - 1:50,000, WB: 1:500 - 1:10,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Smad3 (also known as Mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, Mad3, hMAD-3, JV15-2 or hSMAD3) is a transcriptional modulator activated by TGF-b (transforming growth factor) and activin type I receptor kinase.  These activators exert diverse effects on a wide array of cellular processes.  The Smad proteins mediate much of the signaling responses induced by the TGF-b superfamily.  Briefly, activin type I receptor kinase phosphorylates receptor-activated Smads (R-Smads) at the two extreme serines in the C-terminal SSXS motif; e.g. Smad2 and Smad3 proteins in the TGF-b pathway, or Smad1, Smad5 or Smad8 in the Bone Morphogenetic Proteins (BMP) pathway.  The phosphorylated R-Smad then translocates into the nucleus, where it regulates transcription of target genes.  Based on microarray and animal model experiments, Smad3 accounts for at least 80% of all TGF-b-mediated responses.

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot using affinity purified anti-Smad3 to detect over-expressed Smad3 in 231 cells (lane 2). Lane 1 shows mock infection of 231 cells with lentiviral vector alone. The membrane was probed with the primary antibody at a 1:5,000 dilution. Personal Communication, Allan Weissman, CCR-NCI, Bethesda, MD. Personal Communication


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