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Anti-B2M Antibody


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SKU Description Amount Price Availability*  
  • Rabbit polyclonal Beta-2-Microglobulin antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401311) , 20ug Explanation
500ug $325 3-7 Days
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OriGene Data

ImmunogenAnti-Beta-2-Microglobulin Antibody was produced by repeated immunizations with human beta-2-Microglobulin protein isolated from urine.
Clone Name IsotypeIgG
Species Reactivityhuman Concentration5.0 mg/mL
Guaranteed Application *IHC, IF Suggested DilutionsELISA: 1:2,000 - 1:10,000, WB: 1:1,000 - 1:5,000, IHC: 1:500 - 1:2,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Anti-beta-2-Microglobulin Antibody detects beta-2-Microglobulin. Beta-2-microglobulin is a component of the class I major histocompatibility complex (MHC), which are present on all nucleated cells (excludes red blood cells). It is involved in the presentation of peptide antigens to the immune system. Beta-2-microglobulin associates not only with the alpha chain of MHC class I molecules, but also with class I-like molecules such as CD1 and Qa. Defects in B2M are the cause of hypercatabolic hypoproteinemia. Anti-beta-2-Microglobulin Antibody is ideal for investigators involved in Cell Signaling, Immunology and Cell Biology research.

Reference Data

Target NameHomo sapiens beta-2-microglobulin (B2M)
Alternative Namebeta chain of MHC class I molecules; beta-2-microglobin; beta-2-microglobulin
Database LinkNP_004039
Entrez Gene 567 Human
Related PathwaySecreted ProteinDruggable Genome Antigen processing and presentation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

IHC Image
Immunohistochemistry of Rabbit anti-Beta-2-Microglobulin Antibody. Tissue: normal human skin. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Left panel: isotype control, Right panel: ß2 microglobulin antibody at 1 ug/ml for 20 min at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ß2 microglobulin is cell membrane (and to lesser amount cytoplasmatic compartment). Staining: Beta-2-Microglobulin as brown with diaminobenzidine and with a hematoxylin purple counterstain.
IF Image
Immunofluorescence Microscopy of Rabbit Anti-Beta-2-microglobulin antibody. Tissue: Human Embryonic Kidney cells (HEK 293 cells). Fixation: 2% paraformaldehyde for 15 minutes. Antigen retrieval: not required. Primary antibody: ß-2 microglobulin antibody at 1:200 for overnight at 4°C. Secondary antibody: donkey anti-rabbit secondary antibody conjugated with Alexa Fluor 488 fluorochrome at 1:1,000 for 45 min at RT. Localization: ß2-microglobulin is mainly on the cell surface and occasionally cytoplasmic. Staining: Beta-2-microglobulin as green fluorescent signal.


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