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Anti-PCNA Antibody


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SKU Description Amount Price Availability*  
TA319162 Rabbit polyclonal anti-PCNA antibody 100ug $325 3-7 Days
LC400929 PCNA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart


OriGene Data

ImmunogenThis affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human PCNA protein.
Clone Name IsotypeIgG
Species Reactivityhuman, monkey, dog, mouse, rat, bovine, chicken, fish, Xenopus Concentration1.69 mg/mL
Guaranteed Application *WB Suggested DilutionsELISA: 1:2,000 - 1:8,000, WB: 1:500 - 1:2,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note The proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the processibility of DNA polymerase during elongation of the leading strand.  PCNA is expressed in the nucleus of all proliferating cells and is pivotal for DNA synthesis and cell cycle progression. In response to DNA damage, PCNA is mono-ubiquitinated and is involved in mismatch-provoked excision.  PCNA is a useful marker for DNA synthesis and is highly conserved among most species.

Reference Data

Target NameHomo sapiens proliferating cell nuclear antigen (PCNA), transcript variant 1
Alternative NameATLD2
Database LinkNP_002583
Entrez Gene 5111 Human
Entrez Gene 18538 Mouse
Entrez Gene 25737 Rat
Entrez Gene 718006 Monkey
Entrez Gene 477166 Dog
Related PathwayStem cell - PluripotencyDruggable Genome DNA replicationBase excision repairNucleotide excision repairMismatch repairCell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot using affinity purified anti-PCNA antibody shows detection of PCNA protein in HEK293 (lane 1) and Jurkat (lane 2) whole cell extracts. Approximately 25 ug of lysate was loaded per lane onto a 4-20% gradient gel followed by transfer to nitrocellulose. After blocking, the membrane was incubated with the primary antibody diluted to 1:1000. The membrane was washed and reacted with a 1:10,000 dilution of IRDye® 800 Conjugated Affinity Purified Goat-anti-Rabbit IgG [H&L] MX10 (611-132-122) (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers indicated at the right (lane M, 700 nm channel, red). Other detection systems will yield similar results.


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